lambda dg-4 150 w xenon light source Search Results


150 w xenon lamp  (Sutter Instrument)
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Sutter Instrument 150 w xenon lamp
150 W Xenon Lamp, supplied by Sutter Instrument, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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150 w xenon arc lamp lambda dg-4  (Sutter Instrument Company)
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Sutter Instrument Company 150 w xenon arc lamp lambda dg-4
150 W Xenon Arc Lamp Lambda Dg 4, supplied by Sutter Instrument Company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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150w xenon, lambda dg-4  (Sutter Instrument Company)
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Sutter Instrument Company 150w xenon, lambda dg-4
150w Xenon, Lambda Dg 4, supplied by Sutter Instrument Company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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inverted microscope diaphot 200  (Nikon)
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Nikon inverted microscope diaphot 200
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filter set 31001  (Chroma Technology Corporation)
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microscope  (Nikon)
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Nikon microscope
Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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epifluorescence te 200 inverted microscope  (Nikon)
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metafluor software  (universal imaging inc)
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fluo-4 am  (Thermo Fisher)
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Thermo Fisher fluo-4 am
The alkalinization-induced [Ca2+]i decrease is not mediated by plasma membrane influx or extrusion. A: an intact rod loaded with <t>Fluo-4</t> and nitrophenyl (NP)-EGTA (20 μM). Regions of interests were placed on the cell body (solid trace) and the outer segment (OS; dotted trace) of the cell. [Ca2+]i was elevated by a 5-ms white flash (h v, arrow). In the IS, [Ca2+]i remained at a sustained [Ca2+]i plateau due to suppression of plasma membrane Ca2+ ATPases-mediated Ca2+ extrusion with 1.5 mM La3+. With all IS plasma membrane influx/extrusion mechanism blocked, NH4Cl still evoked a reversible decrease in [Ca2+]i. Subsequent permeabilization with ionomycin rapidly decreased [Ca2+]i. B: same protocol, cone IS. Uncaging flashes (h v, arrows) evoked [Ca2+]i increases in the presence of 1.5 mM La3+ and thapsigargin (1 μM). Stimulation with Na+ acetate (10 mM) increased, whereas NH4Cl (15 mM) decreased [Ca2+]i. C: summary of data from NP-EGTA/Fluo-4-loaded cells stimulated with NH4Cl in the presence of La3+ (*P < 0.05; **P < 0.001).
Fluo 4 Am, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lambda dg4 light source 150 w  (Sutter Instrument Company)
90
Sutter Instrument Company lambda dg4 light source 150 w
The alkalinization-induced [Ca2+]i decrease is not mediated by plasma membrane influx or extrusion. A: an intact rod loaded with <t>Fluo-4</t> and nitrophenyl (NP)-EGTA (20 μM). Regions of interests were placed on the cell body (solid trace) and the outer segment (OS; dotted trace) of the cell. [Ca2+]i was elevated by a 5-ms white flash (h v, arrow). In the IS, [Ca2+]i remained at a sustained [Ca2+]i plateau due to suppression of plasma membrane Ca2+ ATPases-mediated Ca2+ extrusion with 1.5 mM La3+. With all IS plasma membrane influx/extrusion mechanism blocked, NH4Cl still evoked a reversible decrease in [Ca2+]i. Subsequent permeabilization with ionomycin rapidly decreased [Ca2+]i. B: same protocol, cone IS. Uncaging flashes (h v, arrows) evoked [Ca2+]i increases in the presence of 1.5 mM La3+ and thapsigargin (1 μM). Stimulation with Na+ acetate (10 mM) increased, whereas NH4Cl (15 mM) decreased [Ca2+]i. C: summary of data from NP-EGTA/Fluo-4-loaded cells stimulated with NH4Cl in the presence of La3+ (*P < 0.05; **P < 0.001).
Lambda Dg4 Light Source 150 W, supplied by Sutter Instrument Company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


The alkalinization-induced [Ca2+]i decrease is not mediated by plasma membrane influx or extrusion. A: an intact rod loaded with Fluo-4 and nitrophenyl (NP)-EGTA (20 μM). Regions of interests were placed on the cell body (solid trace) and the outer segment (OS; dotted trace) of the cell. [Ca2+]i was elevated by a 5-ms white flash (h v, arrow). In the IS, [Ca2+]i remained at a sustained [Ca2+]i plateau due to suppression of plasma membrane Ca2+ ATPases-mediated Ca2+ extrusion with 1.5 mM La3+. With all IS plasma membrane influx/extrusion mechanism blocked, NH4Cl still evoked a reversible decrease in [Ca2+]i. Subsequent permeabilization with ionomycin rapidly decreased [Ca2+]i. B: same protocol, cone IS. Uncaging flashes (h v, arrows) evoked [Ca2+]i increases in the presence of 1.5 mM La3+ and thapsigargin (1 μM). Stimulation with Na+ acetate (10 mM) increased, whereas NH4Cl (15 mM) decreased [Ca2+]i. C: summary of data from NP-EGTA/Fluo-4-loaded cells stimulated with NH4Cl in the presence of La3+ (*P < 0.05; **P < 0.001).

Journal: American Journal of Physiology - Cell Physiology

Article Title: Intracellular pH modulates inner segment calcium homeostasis in vertebrate photoreceptors

doi: 10.1152/ajpcell.00264.2010

Figure Lengend Snippet: The alkalinization-induced [Ca2+]i decrease is not mediated by plasma membrane influx or extrusion. A: an intact rod loaded with Fluo-4 and nitrophenyl (NP)-EGTA (20 μM). Regions of interests were placed on the cell body (solid trace) and the outer segment (OS; dotted trace) of the cell. [Ca2+]i was elevated by a 5-ms white flash (h v, arrow). In the IS, [Ca2+]i remained at a sustained [Ca2+]i plateau due to suppression of plasma membrane Ca2+ ATPases-mediated Ca2+ extrusion with 1.5 mM La3+. With all IS plasma membrane influx/extrusion mechanism blocked, NH4Cl still evoked a reversible decrease in [Ca2+]i. Subsequent permeabilization with ionomycin rapidly decreased [Ca2+]i. B: same protocol, cone IS. Uncaging flashes (h v, arrows) evoked [Ca2+]i increases in the presence of 1.5 mM La3+ and thapsigargin (1 μM). Stimulation with Na+ acetate (10 mM) increased, whereas NH4Cl (15 mM) decreased [Ca2+]i. C: summary of data from NP-EGTA/Fluo-4-loaded cells stimulated with NH4Cl in the presence of La3+ (*P < 0.05; **P < 0.001).

Article Snippet: For uncaging experiments, cells were incubated simultaneously with 20 μM of nitrophenyl (NP)-EGTA AM and 5 μM Fluo-4 AM (Invitrogen) for 20 min; Ca 2+ was uncaged by 5-ms flashes of white light (340–700 nm broadband excitation at 150 W, Lambda DG4; Sutter Instruments) delivered via the liquid light guide from the Xenon arc lamp.

Techniques: